Evaluation of the Sample in Smears and Liquid-Based Preparations
Fig. 5.2 Flat sheet of parabasal cells
with preserved nuclear polarity in
atrophy. Thinprep (papanicolaou x Mp).
Fig. 5.4 Koilocytes
showing perinuclear cavitation and binucleation in LSIL.
thinprep (papanicolaou x Mp).
Fig. 5.3 Lymphoid cells appear in aggregates
in chronic lymphocytic
cervicitis. Thinprep (papanicolaou x Mp).
Single cells with high n/c ratio and hyperchromasia in
Surepath (papanicolaou x Mp).
Key features of low-grade squamous intraepithelial
• Large cell size;
• Multinucleation; and
• Nuclei show decreased hyperchromasia (Fig. 5.4).
Key features of high-grade squamous intraepithelial
• Fewer abnormal cells;
• Single cells more common than sheets;
• Syncytial aggregates;
• High nuclear cytoplasmic ratio; and
• Nuclear membrane irregularities (Figs. 5.5, 5.6) .
Key features of squamous cell carcinoma
• Single cells and syncytial aggregates;
• Pleomorphic and less hyperchromatic nuclei;
• Rare nucleoli; and
• Diathesis surround cells or cling to malignant cells
(Figs. 5.7, 5.8).
Key features of endocervical adenocarcinoma in situ
• Strips of cells with pseudostratification;
• Nuclear crowding; and
• Subtle appearance of feathering and rosettes.
Key features of endometrial cells
• Tight or lose cell clusters;
• Vacuolated cytoplasm;
• Enhanced nuclear detail; and
• Confused with low-grade endometrial adenocarci-
noma (Fig. 5.9).
Key features of endometrial adenocarcinoma
• Papillary configurations;
• 3D groups; and
• Less prominent tumor diathesis (Fig. 5.10) .